Applications of the CRISPR-Cas9 system

Applications of the CRISPR-Cas9 system

Nowadays, there are many applications that apply the CRISPR-Cas9 tools, including gene knockout, knockin SNPs, insertions and deletions in cell lines and animals. If you interest, visiting the website http://www.origene.com/CRISPR-CAS9/. Here, they are some applications of CRISPR/Cas9 for therapeutics,

CRISPR/Cas9 for promising treatment of HIV infection

Currently, there are many researches study about how to use the CRISPR/Cas9 as a tool to fight HIV that infects the cell.

Learn more in article: 

-HIV overcomes CRISPR gene-editing attack
-CRISPR/Cas9-Derived Mutations Both Inhibit HIV-1 Replication and Accelerate Viral Escape
-In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models

CRISPR/Cas for promising treatment of cancer

CRISPR/Cas could be used as the tool to study the cancer for therapy.

Learn more in article:

-Targeting genomic rearrangements in tumor cells through Cas9-mediated insertion of a suicide gene

CRISPR/Cas for development the antimicrobials

CRISPR/Cas could be used as the tool to kill the specific bacteria that are harmful but not affect the harmless bacteria. The application bases on the process of bacteria that generate the short RNA sequence to guide the Cas9 to fight the bacteriophage by removing its DNA [1]. Here, they are some articles that employ the CRISPR/Cas tool in study of antimicrobials, 

Learn more in article: 

-Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials

-Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases 


References

1. https://www.nature.com/news/antibiotic-alternatives-rev-up-bacterial-arms-race-1.17621

2. The page of Biology Beyond Nature: ชีววิทยาเหนือธรรมชาติ on facebook